Foci Counter

In the realm of cellular analysis, foci quantification stands out as a critical process, shedding light on the intricate details of cellular responses, particularly in the context of DNA damage and repair. Foci, localized concentrations of proteins or other molecular markers, serve as indicators of specific cellular events, with DNA double strand breaks being a prominent example. Such breaks could be initiated via irradiation from the singletron accelerator in CIBA and studied in a controlled manner, providing valuable insights to the damage and repair mechanisms of cells.

The process of foci quantification involves the systematic counting and analysis of these concentrated points within a cell or nucleus. This quantification is often carried out using advanced imaging techniques, such as high resolution laser scanning 3D confocal microscopy, where specific markers or dyes highlight the foci for precise identification and analysis.

In the context of DNA double strand break analysis, foci quantification becomes especially crucial. Automated methods, often integrated with image processing algorithms, enable the efficient and accurate identification of these foci, providing us with quantitative data on the extent of DNA damage. Parameters such as foci counts, volumetric properties, and intensity variations are meticulously assessed, offering a comprehensive view of the cellular response to various stimuli, such as radiation or genotoxic stress.